Fig. 4

Immunological features of T cells. A, Sub-clustering of T cells. Each dot corresponds to a single cell, colored according to cell type. B, The expression distribution of canonical cell marker genes in the identified 12 T cell subsets, shown by Violin plots. C, UMAP projection of T cell subsets among Control, E-NoSEP, Y-SEP, E-SEP conditions. D, The bar plot shows the average proportion of each T cell subset derived from four conditions. E, The percentage of 7 identified T cell subsets across four conditions. Cytotoxic CD8+T cells were excluded in the following analysis. Conditions are shown in different colors. Horizontal lines represent median values, with whiskers extending to the furthest data point within a maximum of 1.5 × interquartile range. All differences with P < 0.05 are indicated. *P < 0.05, **P < 0.01; T-test was used for analysis. F, Box plots of the apoptosis score across different clusters and conditions. Conditions are shown in different colors. Horizontal lines represent median values, with whiskers extending to the furthest data point within a maximum of 1.5 × interquartile range. All differences with P < 0.05 are indicated. ****P < 0.0001; T-test was used for analysis. G, Box plots of the cytotoxicity score similar to F. ****P < 0.0001; T-test was used for analysis. H, Box plots of the exhaustion score similar to F. **P < 0.01, ***P < 0.001, ****P < 0.0001; T-test was used for analysis. I, Scatter-plot showing 49 DEGs in T cells of Y-SEP (n = 3) or E-SEP patients (n = 3) in comparison with Control (n = 3). Each red dot represents an individual gene with Wilcox test adjusted P value ≤0.01 and average log2 fold change ≥0.25 in the Y-SEP/Control and E-SEP/Control comparisons. J, Representative GO-BP terms enriched in the DEGs which are colored in red as in I. A redder one indicates a bigger P value. The top 20 enriched GO terms are shown. K, Circos plot showing the selected enriched KEGG pathway in DEGs of T cells between E-SEP and Y-SEP. The top chord widths represent different T cell subsets. The bottom chord widths represent different KEGG pathways which are shown in different colors