Fig. 5
From: Single-cell landscape of immunological responses in elderly patients with sepsis
Expanded TCR clones and usage of V(D)J genes A, UMAP of T cells derived from PBMCs. Clusters are shown in different colors (left) and TCR detection (right). B, The distribution of clone status of T cells in each condition. Clonal represents clonotype counts ≥2, no clonal represents clonotype counts=1, non-VDJ means cells without TCR. C, Bar plots showing the clonotype proportion in each condition. Conditions are shown in different colors. D, Bar plots showing the percentage of TCR in each T cell cluster. E, The association between the number of T cell clones and the number of cells per clonotype. The dashed line separates nonclonal and clonal cells. LOESS fitting is labeled as the solid line showing negative correlation between the two axes. F, Bar plots showing the clonotype proportion in each sample. Samples are shown in different colors. G, The distribution of clone status of T cell subsets across four conditions. H, Pie plots showing the clonal status percentage of T cells (left) and TCR clone differences across the Control, E-NoSEP, Y-SEP, E-SEP conditions. I, The top ten CDR3 usages are shown. Each bar is colored by condition identity