Fig. 6

Trajectory and pseudotime analysis of CD4+T cells and expression of functional genes in Tregs. A, Trajectory analysis of CD4+T cell subsets color-coded by annotated cell subsets. B, Trajectory color-coded by pseudotime. C, Pseudotime analysis of CD4+T subsets color-coded by cell type. D, Trajectories of CD4+T cells across four conditions color-coded by cell type. E, Trajectory color-coded by cell state. The pseudotime trajectory showed 5 different states of CD4+T cells. F, Trajectories of CD4+T cells across four conditions color-coded by cell state. G, Proportions of 5 cell states in four conditions. Conditions are shown in different colors. H, Heatmap showing the clustering and expression kinetics of top 50 DEGs in branch 1. I, Heatmap showing the clustering and expression kinetics of top 50 DEGs in branch 2. J, Relative expression kinetics of FOXP3, TIGIT, CTLA4, and PDCD1 over pseudo-time across four conditions. K, Dot plots showing the scaled expression of key functional genes, such as FOXP3, TIGIT, TNFRSF18, and IL2RA, in Tregs across four conditions. The color key from blue to red indicates low to high expression levels. The dot size indicates the percentage of cells that expressed genes