Fig. 6

Gene expression changes induced by non-neurotropic IAV infection in young and old mice show different diseases and functions responses. Changes in gene expression in brains of uninfected (mock) young and old mice, and mice 3 and 7 days after IAV infection were quantified by NanoString nSolver 4.0 software and significance in infected mice compared with age-specific uninfected mice was determined by multiple t-test with a Benjamini-Hochberg post-test. Gene lists with associated data were analyzed using Ingenuity Pathway Analysis (Qiagen). Results show Diseases or Functions annotation z-scores in young mice (A) and old mice (B). Only results with z-score ≤ -2.0 or ≥ 2.0 suggesting inhibition (designated blue and -) or activation (designated red and +), respectively, at either Day 3 or Day 7 p.i. are shown. Shading indicates similar diseases or functions in young and old mice. Mouse numbers in each group are shown in Fig. 1A