Fig. 3
Melatonin treatment enhances the maturation of NK cells in the spleen and bone marrow of aged mice. (A) Following melatonin treatment, flow cytometry was employed to observe the progression of NK cell development, with CD27 and CD11b serving as markers for distinct developmental stages of NK cells. (B) Statistical graph illustrating the proportion of NK Cell subsets in the spleen and bone marrow of mice across various developmental stages. (C) Statistical graph depicting the absolute numbers of NK cell subsets in the spleen and bone marrow of mice across various developmental stages. (D) Following melatonin treatment, NK cell development was assessed using a flow cytometer, with NK1.1 and CD11b serving as markers for distinct developmental stages of NK cells. (E) Statistical graph representing the proportion of NK cell subsets in the spleen and bone marrow of mice across various developmental stages. (F) Statistics depicting the absolute numbers of NK cell subsets across different developmental stages in mouse spleen and bone marrow. The data is presented as means ± SD. n = 6. Unpaired two-tailed Student’s t-tests were conducted using Prism software. A p-value of less than 0.05 was deemed statistically significant. *P < 0.05, **P < 0.01