Fig. 4

In vivo melatonin treatment enhances the capacity of NK cells in aged mice to exhibit CD107a expression and IFN-γ secretion. (A) Following melatonin treatment, spleen lymphocyte cells are collected. Subsequently, upon stimulation with RMA-S and YAC-1 cells, the secretion of CD107a is measured through flow cytometry. (B) Statistical chart representing CD107a secretion by NK cells. (C) Following melatonin treatment, spleen lymphocyte cells were collected. Subsequent to stimulation with RMA-S and YAC-1 cells, flow cytometry was employed to analyze the secretion of IFN-γ. (D) Statistical chart depicting IFN-γ secretion by NK Cells. The data is presented as means ± SD. n = 6. Unpaired two-tailed Student’s t-tests were conducted using Prism software. A p-value of less than 0.05 was considered statistically significant. *P < 0.05, **P < 0.01, ***P < 0.001