Fig. 5

Melatonin stimulates the proliferation of CD11b single-positive NK cells in the spleen of aged mice. (A) Following melatonin treatment, flow cytometry was utilized to assess the expression of Ki67 in various developmental stages of NK cells. (B) Statistics of Ki67-positive cells in various subgroups of spleen NK Cells. (C) Following melatonin treatment, flow cytometry was employed to detect the expression of Annexin V in NK cell subgroups at different developmental stages. (D) Statistical Chart Depicting Annexin V-Positive Cells in Different Subgroups of Spleen NK Cells. The data is presented as means ± SD. n = 6. Unpaired two-tailed Student’s t-tests were conducted using Prism software. A p-value of less than 0.05 was considered statistically significant. *P < 0.05, **P < 0.01