Fig. 5

Aged mice exhibit increased inflammasome activation in the brain in response to endotoxin challenge. Representative image of a mouse 6 months following skull-thinning cranial window surgery (A). Young (6–12 week old) and aged (18 month – 2 year old) mice were challenged with LPS (100 μg, i.p). Representative in vivo IVIS images prior to (0 h) and 24 h-post LPS administration (B). C. Brains were excised from young (3–5 month old) and aged (22 – 23.5 month old) caspase-1 biosensor mice and 400-450 μm coronal sections were generated (~ 25–45 slices per group). Slices were treated with and without LPS and imaged using the IVIS (D). (C-D) N = 6–10 animals per group, * = p