Fig. 2

CD3 + T cell populations. A Cluster partitions by FLOWSOM of PBMCs stained with antibodies for CD3 + T-cell markers. In total, 19 different clusters were defined (left). OptSNE plots visualizing contour plots of the 3 patient groups (right). B Frequencies of T cell populations in young, old and old-frail patient groups. Cell populations are presented as percentage of the total CD45 + cells. Statistical differences were assessed with Kruskall-Wallis tests and p < 0.05. C Expression levels of each of the indicated markers is depicted for the individual immune cell populations