Fig. 2
From: Ageing results in an exacerbated inflammatory response to LPS by resident lung cells
Inflammatory cell numbers in the airways and lung following LPS challenge of young and aged mice. Inflammatory cell numbers in (a) airway BAL fluid and (b) lung tissue were determined by flow cytometry 4 h after challenge with 100ng LPS or PBS vehicle in young and aged mice. Each data point represents an individual mouse and bars show mean +/- SEM (n = 5). Filled circles = young and open circles = aged mice. Data are representative of two independent experiments. Significance was determined by two-way ANOVA, comparing each mean with others in its age group and with others in the LPS or PBS groups. (c) Differential counts of airway cells obtained by bronchoalveolar lavage of young and aged mice 4 h after LPS challenge counted on cytospin preparations (n = 5). Significance was determined by student’s t-test. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001