Fig. 1
Comparison of extracellular vesicles isolated from young and old mouse plasma
(a) Schematic diagram of project experimental design. Y_EV = young mouse plasma extracellular vesicle, O_EV = old mouse plasma extracellular vesicle, Y_BMDM = young mouse bone marrow-derived macrophage, O_BMDM = old mouse bone marrow derived-macrophage. The table shows the factorial experimental design. (b) Mean diameter of Y_EV and O_EVs, determined by NTA. (c) Mode diameter of Y_EVs and O_EVs, determined by NTA. (d) Total particle concentration of Y_EVs and O_EVs, determined by NTA. (e) High magnification and low magnification cryoEM images of Y_EV and O_EV isolates, with representative NTA peaks. EVs are indicated by arrows in the low magnification image. (f) Total protein concentrations of Y_EV and O_EV isolates. (g) Particle: protein ratio for Y_EV and O_EV isolates. (h) Total protein concentration of young (Y) and old (O) mice plasma. Each data point represents EVs isolated from a separate animal. Bar heights represent the mean and error bars show standard error of the mean. Y_EVs were compared to O_EVs by unpaired two-way t-test. ns = not significant (P > 0.05), * = P ≤ 0.05, ** = P < 0.001