Fig. 3
Upregulation of p16 causes cell cycle arrest in BMMCs. a, b. control (a) and p16 inducible (b) BMMCs were seeded at a concentration of 1 × 106 cells/ml in the absence or presence of DOX. Cells were collected and counted at the indicated time periods. c. Cell viability of p16 inducible BMMCs following a 4-day incubation with or without DOX measured by quantifying the incidence of DAPI-negative cells using flow cytometry. d. Cell viability of 5 × 104 p16 inducible BMMCs following 4 days incubation with or without DOX and measured with an MTT assay. e. Representative flow cytometric analysis of the cell cycle of p16 inducible BMMCs following a 3-day incubation with or without DOX. Data is presented as means ± SEM. Statistical significance was determined by Mann-Whitney (b) or unpaired t-test (a–e) n = 3–5 experiments