Fig. 9
Senescence enhances regulated release of small CD63-positive EVs. (a) Representative dot blot and densitometric quantification of EVs released by untreated (UT) or IgE/Ag-activated 2 × 106 p16 inducible or control BMMCs following a short-term incubation with or without DOX and staining for CD63. (b) Representative dot blots and densitometric quantification of cell lysates of UT or IgE/Ag-activated p16 inducible BMMCs following a short-term incubation with or without DOX and staining for CD63 and with anti-total ERK2 for normalization. c, d. Nanoparticle Tracking Analysis of EVs’ concentration and size (c) or concentration of 30–150 nm sized EVs (d) released by 2 × 106 IgE/Ag-triggered p16 inducible BMMCs following a short-term incubation with or without DOX. Data is presented as means ± SEM. Statistical significance was determined by either Welch’s (a) or (un)paired t-test (a–d). n = 3–5 experiments