Fig. 9

Elocalcitol mitigates HFD-induced behavioral and hypothalamic senescence changes in WT mice but not in Mir146a-/- Mice. (A) Body weight changes across four groups: WT HFD, Mir146a-/- HFD, WT HFD + Elo, and Mir146a-/- HFD + Elo. (B) Preference ratio for the novel object in the novel object recognition test for the same groups. (C, D) RT-qPCR analysis of p16 and p21 mRNA expression levels. (E) Flow cytometry analysis of β-galactosidase positive microglial cells in the hypothalamus. (F-H) RT-qPCR analysis of SASP cytokines in the hypothalamus of WT and Mir146a-/- mice on HFD and Elo treatment. Number of animals = 4–10. Data panels (A, C, D, E, G) did not show normal distribution and were transformed into logarithms (Y = log(Y) and analyzed using one-way ANOVA with Tukey’s multiple comparisons test and panels (B, F, H) are processed using two-way ANOVA followed by Tukey’s multiple comparisons test. Data are represented as mean ± SEM; * p < 0.05, ** p < 0.01, **** p < 0.0001