Fig. 3

Transcriptome difference analysis of the mPFC between AU and AI rats. (A) PCA plot. PC1, the first principal component; PC2, the second principal component. (B) Volcano plot of DEGs in each group. Y-axis: adjusted P value; x-axis: log2-fold change (log2FC). The red dots represent genes whose expression was upregulated more than log2(2), the blue dots represent genes whose expression was downregulated less than log2(0.5), and the black dots represent genes whose expression was not significantly different. (C) GO term enrichment analysis. x-axis: adjusted P value. (D) GSEA of all genes. P values < 0.05 were considered statistically significant