Fig. 5

The potential mechanisms among ALDH1L1, mitochondrial dysfunction and inflammatory response. a. Flow cytometry histograms of MitoSOX-positive HCT116 cells (left), percentage of MitoSOX-positive cells relative to NC (middle), quantification of MitoSOX-positive MFI relative to NC (right) in each group (N = 3). b. WB showed the changes of NLRP3 and IL-1β in each group compared with the negative controls (N = 4). c. The OCR measurement of each group (left). The boxplots illustrated basal respiration, maximal respiration, spare respiration capacity, non-mitochondrial respiration and ATP production in each group (N = 3). Data are mean ± SEM of biological triplicates, and are representative of 3 independent experiments, analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test (a, c) or Kruskal–Wallis test (b). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Abbreviation: NC, negative control; MFI, mean fluorescence intensity; OCR, oxygen consumption rate