Fig. 13

Three months ingestion of high fat diet (HFD) robustly alters colon microbiome composition dependent of age. A Alpha-diversity indices as measured by Faith’s PD. B Four top bacterial taxa (represented as % total bacteria) upregulated (Lactococcus, Blautia) and downregulated (Turicibacter, Clostridia UCG0-14) by HFD independent of age. C Two factor negative binomial (NEGBIN) reveals age x diet interactions for four bacterial taxa (adjusted p < 0.05). D Heatmap representing Spearman correlation coefficients comparing bacterial genera abundance vs. brain region specific protein levels in the aged group. * p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001