Fig. 5

Microglia are increased in the aged retina. Young (3-4mo) and aged (18-26mo) WT and µs-/- neural retinas were isolated and processed to a single cell suspension. (A) Representative FACS plots show gating on CD45 + cells. The average percent of retinal CD45 + cells is shown. (B) Representative FACS plots show gating for CD45⁺CD11b⁺ microglia. (C) Graphs showing the total percent of CD45 + cells found in retinal cell preps, the percent of CD45 + CD11b + microglia, and median fluorescent intensity (MFI) of CD86 and CD206. FMO, fluorescence minus one. Total Retinas: young WT n = 12; Aged WT n = 28; young µs-/- n = 12; aged µs-/- n = 26. Results based on two independent experiments with retinas from individual mice pooled in each of the two experiments. Statistics: ANOVA with Kruskal Wallis post-hoc analysis; *p < 0.05,**p < 0.01,***p < 0.001