Table 2 Primary and secondary antibodies used
Primary Antibody | Dilution | Source | Secondary Antibody | Source |
|---|---|---|---|---|
Goat anti-Iba-1 | 7.5 µl/mL | Abcam; ab98887 | Donkey anti-goat AF405 or AF 488 | Abcam; ab175700 |
Chicken anti-GFAP | 3.4 µl/mL | Abcam; ab4674 | Donkey anti-chicken AF647 | Jackson; #703-605-155 |
Rabbit anti-IL-1β | 10 µl/mL | Abcam; ab205924 | Donkey anti-rabbit AF488 or AF568 | Abcam; ab150073 |
Rabbit anti-Caspase-3 | 3.4 µl/mL | Abcam; ab44976 | Donkey anti-rabbit AF488 or AF568 | Abcam; ab150073 |
Rabbit anti-Thy1.2/CD90 | 3.4 µl/mL | Abcam; ab273071 | Donkey anti-rabbit AF488 or AF568 | Abcam; ab150073 |