Fig. 2

B cell subsets in naturally aged vs. HSC-transplanted mice. The number of CD19⁺ B cells in the spleen of DY-HSC and DO-HSC transplanted hosts at 18 weeks post transplantation (n = 6) (a) and of young (Y; 3 months) and old (O; 22–24 months) mice (n = 10–12) (b) was determined by flow cytometry. (c-f) The numbers of different B cell subsets such as follicular B cells (FOB), marginal zone B cells (MZB), age-associated B cells (ABC) (c, d), and of IgD⁻ B cell subsets such as antibody secreting B cells (ASC), class switched B cells, germinal center B cells (GC B) and switched memory B cells (SMB) (e, f) was determined in the spleen of DY-HSC and DO-HSC hosts (n = 3–4) (c, e) and of young (Y) and old (O) mice (n = 6–10) (d, f) and was determined by flow cytometry. A representative HSC-transplantation experiment out of three experiments is shown in panels a, c and e. Statistical significance between the indicated B cell populations was determined using the unpaired students t-test. p < 0.05*, p < 0.01**, p < 0.001***. ns, not significant. Mean values±SD are shown